CONFOCALMICROSCOPY Archives

September 1995

CONFOCALMICROSCOPY@LISTS.UMN.EDU
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Subject:
Re: Green Fluorescent Protein
From:
Matthew Hannah <[log in to unmask]>
Reply To:
Matthew Hannah <[log in to unmask]>
Date:
Wed, 20 Sep 1995 19:01:38 +0200
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In message  <Pine.PCP.3.90.950920084344.8621A-100000@[142.150.128.115]> Confocal
Microscopy List writes:
> Hello there,
>
> Did anyone try to fix Green Fluorescent Protein expressed
> intracellularly?   Any feeling which fix is a good one and which is a NO,
 
There is someone here using GFP expressed in the secretory pathway (Kaether &
Gerdes, Febs Letts. 369. 267-271). He uses 3% paraformaldehyde which appears to
work fine.
 
Matthew
 
Dr. Matthew Hannah
Institute for Neurobiology,
Im Neuenheimer feld 364
D-69120 Heidelberg, Germany
WWW: http://129.206.50.220/Neurobiology.html
Tel: ++49 (6221) 56-3820
Fax: ++49 (6221) 56-3700

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