CONFOCALMICROSCOPY Archives

March 2019

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
Zdenek Svindrych <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 28 Mar 2019 17:01:25 -0400
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Hi Steve,

as mentioned, it could be condensation dissolving some dirt on the glass
and then evaporating.

Also, these cans are full of liquid, and if you're not careful enough
(shaking the can or not holding it upright, especially when full), the
liquid is expelled and can leave residue. Some cans also contain bittering
agent! (I always try to pick the ones that don't, I don't know about the
Newport stuff, try to find an MSDS)

I would just clean it with ethanol swab or tissue or something, and be more
careful next time...

best, zdenek

On Thu, Mar 28, 2019 at 4:30 PM Steven Hou <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi everyone, I saw that the main dichroic that we are using for a
> multiphoton microscope was getting dusty and decided to clean with
> compressed air. The air duster we use is from Newport (
> https://www.newport.com/f/canned-air-duster). Strangely on one of our
> blows, a white area appeared on our filter forming an elliptical shape
> which quickly turned into a white/green-ish halo (see image here:
> https://imgur.com/rlrP0yB) that does not seem to be going away. This is
> on the opposing side of the AR coating.
>
> I was under the impression that using compressed air was a safe way to
> clean optics. Has anyone else encountered a similar problem? Has the
> compressed air damaged the coating in some way?
>
> Thanks,
> Steve
>


-- 
-- 
Zdenek Svindrych, Ph.D.
Research Associate - Imaging Specialist
Department of Biochemistry and Cell Biology
Geisel School of Medicine at Dartmouth

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