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| Date: | Thu, 8 Oct 2009 10:00:46 -0400 |
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We've had good success using the BABB procedure, described here:
http://www.ncbi.nlm.nih.gov/pubmed/16060974
Thomas C. Trusk, PhD
Josh Spruill Imaging Facility
Regenerative Medicine and Cell Biology
MUSC
-----Original Message-----
From: Michael Weber [mailto:[log in to unmask]]
Sent: Wednesday, October 07, 2009 11:56 AM
Subject: optical clearing of tissue
Dear list,
I am looking for advice on optical "clearing" of fixed tissue before
staining it and using it for light microscopy. Actually "tissue" is not
the precise term, since I would like to clear whole fly embryos. This
process seems to be well established in histology, i.e. using Xylene. I
also found a commercial product called "Histo-Clear" (National
Diagnostics), which claims to preserve tissue structures rather well,
while being less nasty compared to Xylene. Did you guys ever use something
like that? Any input welcome.
cheers,
Michael
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