Jim-
Regarding your protocol:
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>Tissue Clearing Procedure for thick slices
>
>  This procedure is used for clearing thick (300-500



µm) slices of rat brain
>tissue which has been fixed with 4% paraformaldehyde  and is stored in
>buffer.
>1. Transfer the tissue to a glass container.
>2. Wash twice with distilled water for 5-10 minutes.
>3. Wash with graded alcohols (65%, 70%, 80%, 85%, 90%, 95% and absolute)
>for 5-10 minutes each.
>4. Wash with xylene for 5-10 minutes.
>5. Wash with Histoclear® for 5-10 minutes.
>6. Mount the tissue in a depression well slide with Methyl Salicylate  and
>seal  the cover slip with clear nail polish.
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Would this be suitable for use with an inverted scope? I have heard (only
anecdotally) that methyl salicylate could solvate nail polish; this would make
me hesitate to work with it inverted over my objectives. Perhaps you can dispel
my misinformation. Or perhaps you use a specific brand of nail polish?

Thanks,

Jonathan Sheehan
Vanderbilt Cell Imaging Core