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April 2005

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Subject:
mounting media for TIRF
From:
"Reece, Jeff (NIH/NIEHS)" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 28 Apr 2005 10:23:34 -0400
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If I want to use a 1.45 NA lens for TIRF (excitation through the lens), but
on fixed specimens in which the mounting medium has dried hard, is there a
mounting medium that would work?  I assume the question is the same as
asking : does anyone know of a mounting medium that dries hard, but
refractive index stays less than ~1.43 (i.e. low enough for TIRF to occur
with this lens)?

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