Brightfield was great,did not get opportunity to try fluorescence,we are buying for stereo scope in a cat2 cabinet.
But for fluorescence they are of interest to us also.
Peter
----- Reply message -----
From: "Watkins, Simon C" <[log in to unmask]>
To: <[log in to unmask]>
Subject: BSL3 microscopy
Date: Fri, Jan 14, 2011 18:50
and did they work peter? (brightfield and fluorescence)
s
Simon C. Watkins Ph.D, FRC Path
Professor and Vice Chair Cell Biology and Physiology
Professor Immunology Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St
Pittsburgh PA 15261
412-352-2277
www.cbi.pitt.edu
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Peter Humphreys
Sent: Friday, January 14, 2011 1:19 PM
To: [log in to unmask]
Subject: Re: BSL3 microscopy
We just demo'd some ivis pieces through Olympus uk if that helps. Peter
Sent from my HTC
----- Reply message -----
From: "Watkins, Simon C" <[log in to unmask]>
To: <[log in to unmask]>
Subject: BSL3 microscopy
Date: Fri, Jan 14, 2011 17:54
*****
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This little question is proving quite fruitful for discussion, this is not a face shield but totally enclosed nonflexible mask with filtered air. to stop any accidents aerosolized or otherwise, the bugs are right on the edge of a level 4 in my mind. we tried screens this am, frosted glass, not enough light. video cameras not enough light, CCD cameras with a reducing adaptor will work but difficult and expensive. The most interesting suggestion so far has been the vision engineering eyepieces.. They superficially look perfect http://www.visioneng.us/isis-ergonomic-microscope-eyepieces-overview.php but.... they stopped selling them 6 months ago... (still on the website, and they are hunting for some)
Simon C. Watkins Ph.D, FRC Path
Professor and Vice Chair Cell Biology and Physiology
Professor Immunology Director Center for Biologic Imaging
BSTS 225
University of Pittsburgh
3500 Terrace St
Pittsburgh PA 15261
412-352-2277
www.cbi.pitt.edu
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Caroline Bass
Sent: Friday, January 14, 2011 11:10 AM
To: [log in to unmask]
Subject: Re: BSL3 microscopy
*****
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Hi,
I've worked in a BSL3 lab before, and did use a general inverted
microscope. Are you sure that the specimen can't be contained and that you
need the face shield? The purpose of a BSL3 facility is to maintain
negative air pressure in case of an incident. A face shield protects
against accidental splashes but doesn't do anything for an aerosolized or
airborne agent (they easily get around a mask). So it seems if there is a
way to contain the specimen either in a sealed environment (flask, or
slide chamber) or to set up a shield between the user and the specimen,
that would serve the same purpose.
Also, what about a monitor situation? If you can contain the specimen from
the user then it seems your typical confocal setup, where most of the work
is done on a computer, contained within the BSL3 facility should be
sufficient.
Caroline Bass
On 1/14/11 9:32 AM, "Watkins, Simon C" <[log in to unmask]> wrote:
>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Folks, I am about to install an advanced imaging system (sweptfield
>confocal and widefield imaging combo) in a biosafety level 3 facility.
>To work in the facility one has to suit up and wear a rigid face mask, as
>the cells/pathogens will be on the scope and may be open to the
>environment. This means that the eyepieces of the scope are essentially
>useless. I wonder if any other listers have dealt with this problem and
>what their solution was? Obviously the widefield camera will help a lot,
>but it doesnt allow survey of the full field of view, as we are doing
>mostly flourescence a video camera isnt much use.... back in the day,
>there were some screen solutions....
>Looking for creative ideas
>S.
>
>Simon C. Watkins Ph.D, FRC Path
>Professor and Vice Chair Cell Biology and Physiology
>Professor Immunology Director Center for Biologic Imaging
>BSTS 225
>University of Pittsburgh
>3500 Terrace St
>Pittsburgh PA 15261
>412-352-2277
>www.cbi.pitt.edu<http://www.cbi.pitt.edu>
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