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Hi Adrian
We have 'recently' (several months) encountered great problems with the Matrical glass bottom 96-well plates. After using them for years without any trouble, the cells suddently started dying (all sorts of cells, any type of medium, in 2D or grown in a gel) as soon as they were seeded. Matrical claimed that they had not changed anything then they blamed the glue but the next batch of plates has the same problem.
Here are a few more companies (thin glass or thin plastic bottom):
MatTek<http://glass-bottom-dishes.com/pages/multiwellproducts.html>
Perkin Elmer<http://www.perkinelmer.com/SE/Catalog/Category/ID/Microplates>
BD<http://catalog.bd.com/bdCat/listProducts.doCustomer?categoryID=R39&priority=25&categoryName=Assay%20Plates%20and%20HTS%20Systems&categoryImgPath=/ecat/images/f44/family/plates.gif&sortByField=Product%20Number&viewPageNum=0>
Ibidi <http://www.ibidi.com/products/disposables/P_8962X_Plate96well.html>
Nexus<http://www.irori.com/pages/technology/keyAttributes.html>
Whatman<http://www.whatman.com/References/GlassBotClearViewMicroDataSheetFINAL3.26.10LR.pdf>
I strongly recommend you ask for a free sample to make sure the plates work for your cells and your imaging system.
Med vänlig hälsning / Best regards
Sylvie
@@@@@@@@@@@@@@@@@@@@@@@@
Sylvie Le Guyader
Live Cell Imaging Unit
Dept of Biosciences and Nutrition
Karolinska Institutet
Novum
14183 Huddinge
Sweden
office: +46 (0) 8 5248 1107
LCI room: +46 (0) 8 5248 1172
mobile: +46 (0) 73 733 5008
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[log in to unmask]] On Behalf Of Eric Griffis
> Sent: 21 June 2012 11:46
> To: [log in to unmask]
> Subject: Re: Glass bottom 96 well plates for hi-res imaging
>
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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Hi Adrian,
>
> I've had great success with plates from Matrical. They sell #1.5 96-well plates with
> square wells, which maximises the number of cells you can image per well. We
> found that they were more consistent than the Whatman plates and my cells grew
> much more happily in these plates.
>
> Best regards,
>
> Eric
>
>
> Eric Griffis
> Principal Investigator
> Wellcome Trust Centre for Gene Regulation and Expression College of Life
> Sciences University of Dundee MSI/WTB/JBC Complex Dow Street Dundee DD1
> 5EH UK
>
> Tel: +44 (0)1382 385118
> Fax: +44 (0)1382 388072
> Email: [log in to unmask]<mailto:[log in to unmask]<mailto:[log in to unmask]:[log in to unmask]>>
> Lab Website:
> http://www.lifesci.dundee.ac.uk/groups/eric_griffis/GriffisLab/index.html
> GRE Website: http://gre.lifesci.dundee.ac.uk
>
>
>
> On 21 Jun 2012, at 10:02, Adrian Smith wrote:
>
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>
> Hi all,
>
> We have a user who is after some 96well plates for hi-res imaging, ie coverslip
> thickness (glass?).
>
> Any comments/recommendations would be appreciated.
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
>
>
>
> The University of Dundee is a registered Scottish Charity, No: SC015096
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