*****
To join or leave the confocal microscopy listserv or to change your email address, go to:
https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
Post images on http://www.imgur.com and include the link in your posting.
*****

Michael, you can try putting a drop of saline or lubricating eye drop on
the cornea and then gently placing a coverslip on the cornea.  You can
gently press it down to flatten the cornea a bit. You will have to move it
around a bit to find the region of the retina you want to image so use a
rectangular coverslip so you have something to hold on to (e.g. 22 mm by 40
mm).  This works well to inspect the retinal morphology with a dissection
scope, but fluorescence might be more challenging. You may need to set your
coverslip up on a manipulator to keep it steady for imaging, rather than
just holding it with your hand.

Hope this works for you.
Sue Keirstead


On Wed, Jul 19, 2023 at 8:27 AM Cammer, Michael <
[log in to unmask]> wrote:

> *****
> To join or leave the confocal microscopy listserv or to change your email
> address, go to:
> https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> This isn't necessarily a confocal question, but is related.
>
> I am working with a lab that needs to image fluorescence protein
> expression in mouse retina over time meaning a picture a day over days.  We
> tried a fluorescence dissection scope, but cannot compensate for the
> lens/cornea, so we cannot focus on the retina.  (Yes, the animal is
> anesthetized, pupil dilated, and protocol approved.)
>
> Of course there are commercial clinical systems for people for visible
> light photography of retinas and could see retrofitting a system for
> fluorescence snapshots of patient retinas with monochrome light and a
> narrow pass filter over the lens.
>
> But any ideas for something for a live mouse in the lab?
>
> Thank you!!
>
>
> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
> NYU Langone Health, 540 First Avenue
> <https://www.google.com/maps/search/540+First+Avenue?entry=gmail&source=g>,
> office MSB 06 57, main lab Smilow C-17, New York, NY  10016
> Office: 646-501-0567 Cell (DO NOT TEXT): 914-309-3270
> [log in to unmask]<mailto:[log in to unmask]>
> http://nyulmc.org/micros  http://microscopynotes.com/
> Scheduling the time you want is far more reliable by phone call.  Why not
> provide your phone number?
>
> Probably nobody reads this part, but everybody should and heed it:
> Acknowledgement in publications and presentations of Microscopy Core
> performed work is vital to secure support and funding necessary to maintain
> this valuable research resource.   For publications that include work
> performed in the core, please use the acknowledgement statement "We thank
> the NYU Langone Microscopy Core for experimental and technical support" and
> include required grant numbers as listed here
> http://microscopynotes.com/ilabnyu/acknowledgements2017.pdf
> Please also consider staff for co-authorship if they played a key role in
> the study.
>
-- 

Susan A. Keirstead, PhD
she, her, hers
Assistant Professor | Integrative Biology and Physiology |
physiology.umn.edu <http://www.physiology.umn.edu>
Administrative Co-director | Stem Cell Institute | stemcell.umn.edu
<http://www.stemcell.umn.edu>
Director of Graduate Studies | Stem Cell Biology  | stemcell.umn.edu
<https://www.stemcell.umn.edu/graduate-programs>/graduate-programs
2-210 McGuire Translational Research Facility
University of Minnesota | umn.edu | 612-626-2290