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That’s what it looks like when the condensate is on the sensor.
For what it's worth, we had this issue with a 6 year old Hamamatsu Orca
Flash4.0 LT (albeit with a smaller area of condensation in the center of
the chip) and they replaced it for $3K. Seems reasonable to me; equivalent
to $500/yr extended warranty I’ve been quoted on similar cameras from other
vendors.
-Esteban
On Thu, Jan 21, 2021 at 7:22 AM Colin Haig <[log in to unmask]>
wrote:
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>
> Hi Vita,
> The "crystals" are very sharply focused, so it is not on the camera sensor
> itself. It is somewhere further down the optical path.
> Ice and frost usually have more definitely fractal edges in my
> experience. Perhaps this is forming around fine particles somewhere in the
> optical setup.
> A dessicant to dry it out and heating to room temperature or a bit above
> might help.
> Check if your camera has a rechargeable desisccant cartridge or
> replaceable desiccant, in case it is forming on an optical window several
> mm in front of the sensor.
> Cleaning may be needed as well.
> Good luck,
> Colin
>
> -----Original Message-----
> From: Confocal Microscopy List <[log in to unmask]> On
> Behalf Of Laurel Ballantyne
> Sent: January 21, 2021 9:01 AM
> To: [log in to unmask]
> Subject: Re: possible crystals on a camera chip
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Vita,
>
> We had a similar problem on a camera. It turned out to be condensation
> which turned to ice. We didn't get the camera repaired, but I turned off
> the camera cooling function and monitored the temperature. I only used it
> for brief periods, so this was a solution for me.
>
> Good luck!!
>
> Laurel
>
> Laurel Ballantyne
> Research Assistant/Lab Manager for Dr. Lindsay Fitzpatrick and Dr. Laura
> Wells Department of Chemical Engineering Queen's University Kingston, ON
> lab/office: HMRC 4-3-302
> website: https://fitzpatricklab.engineering.queensu.ca/
>
> ________________________________
> From: Confocal Microscopy List <[log in to unmask]> on
> behalf of Vita Solovyeva <[log in to unmask]>
> Sent: January 21, 2021 7:48 AM
> To: [log in to unmask] <[log in to unmask]>
> Subject: possible crystals on a camera chip
>
> *****
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>
> Dear All,
>
> I would like to ask your advice about the situation we have with our Leica
> camera (DFC900 GT) on our our life cell Ca-Imaging system.
>
> We noticed that we see crystals in our images (see the images for 20x and
> 100x, made without sample and without oil for 100x, in the attached link
> https://can01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fcloudstorage.elearning.uni-oldenburg.de%2Fs%2F7fMgzCK3XnTGWbo&data=04%7C01%7Cballantl%40QUEENSU.CA%7C5ed7648d33a646d5bffe08d8be0c5457%7Cd61ecb3b38b142d582c4efb2838b925c%7C1%7C0%7C637468307463400452%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C2000&sdata=ytFeP5IBj1%2FougJXBlAP6DmI7ENdJ%2F9Y4gBMx%2BuM1PA%3D&reserved=0
> ).
>
> What I can see:
>
> 1. Crystals size does not change with magnification change (size stays the
> same for 20x, 40x, 63x, 100x).
> 2. When we turn the camera, the crystals do not rotate.
> 3. We can see the crystals even without objective, i.e. we remove an
> objective and have just ambient light shining, we still can see the
> crystals in the software.
> 4. We do not see crystals in the ocular.
> 5. When we opened the camera and looked at it, we could not see anything
> special, no matt film etc.
>
> We asked Leica, they say that it is some crystallisation on the camera
> chip. The repair is about 11000€, new camera is about 16000€.
>
> I would like to ask if you experienced a similar problem, how did you
> solve it?
> Any ideas and suggestions are welcome!
>
> Thank you in advance.
>
> best wishes,
> Vita
>
>
> Dr. Vita Solovyeva
> University of Oldenburg
> Fak V- Institute of Physics
> Carl von Ossietzky-Straße 9-11
> 26129 Oldenburg
> Tel. +49-4417983547
> [log in to unmask]
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