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Mike,

I have two HyDs and I agree w/Vitaly, there is essentially no difference when compared to GaAsP.  We run almost all imaging modalities through deconvolution (Autoquant GPU), and yes there has to be some care taken so that artifacts (present in raw data, ie SA) are not accentuated.  As for quantitative decon see: Calibration of wide-field deconvolution microscopy for quantitative fluorescence imaging. Lee JS; Wee TL; Brown CM. J. of Biomol. Tech. 25(1):31-40, 2014 Apr.

My 2¢


Richard Cole
Research Scientist V
Director: Advanced Light Microscopy & Image Analysis Core 
Wadsworth Center
 
Research Assistant Professor 
Dept. of Biomedical Sciences 
School of Public Health State University of New York 

120 New Scotland Avenue, Albany N.Y. 12208
518-474-7048 Phone
518-408-1730 Fax





>Hi Mike,
>there is no big difference between HyDs and GaAsP detectors. Leica SMD HyDs are cooled to 18 C, thus having ca. 400 dark counts versus 2K or more for the GaAsP.I am >not aware of linear deconvolution algorithms for confocal image data, thus it seems to be "cosmetic" and/or "aesthetic", and not suitable for accurate intensity based >image analysis.Recent integration of FALCON combined with FCS/RICS to the Leica SP8 may bring new era to biology letting biologists quantify their data and image >biological molecules at their physiological concentrations. However, most of biologists seems to be "scared" of basic equations...If you are located in the US, Leica provides >much better Service and Application support compared to Zeiss. I do not have much experience with Olympus and Nikon on the confocal side.
>If you have any specific questions, please let me know.
>VitalyMCCF/MSKCC 

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