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Hi Christian,

If you do sequential scanning you can easily separate CFP, GFP and YFP in
a single plant cell, and have a channel for chlorophyll fluorescence as
well (same pass as GFP).

cheers,
Rosemary

Dr Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia

T 61 2 6246 5475
F 61 2 6246 5334
E [log in to unmask]


On 7/01/12 8:33 PM, "Hanna Sas Nowosielska" <[log in to unmask]>
wrote:

>*****
>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>*****
>
>Dear Christian,
>
>There is a publication by Yuda Fang and David L. Spector "Centromere
>Positioning and Dynamics in Living Arabidopsis Plants" Mol Biol Cell
>(2005)
>Vol. 16, 5710­5718, in which the authors use HTB1-CFP/HTR12-YFP
>transformed
>Arabidopsis plants. Maybe try to contact the authors for the seeds.
>
>All the best,
>Hanna
>
>2012/1/6 Christian <[log in to unmask]>
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> I was wondering if anyone knew where I might find some stable CFP and
>>YFP
>> Arabidopsis seed?  The localization does not matter, I only need to
>> practice with the two proteins in plant material.  I have users coming
>>with
>> "YFP" which is GFP, and a few are starting to use CFP and I'm a bit
>>leery
>> of CFP/GFP crosstalk.  Thusly I have need for practice.
>>
>> Thanks.
>>
>> Christian
>>
>
>
>
>-- 
>Department of Plant Anatomy and Cytology
>Faculty of Biology and Environmental Protection
>University of Silesia
>Jagiellonska Str. 28
>40-032 Katowice
>Poland