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Molecular Probes offers a kit for fixed cell / tissue nuclear labeling
called the "SelectFX Nuclear Labeling Kit", which includes DAPI, SYTOX
Green, 7-AAD (similar in spectrum to PI, but more DNA-selective), and
TO-PRO-3.  The kit includes optimized protocols in the product
information sheet for getting a more DNA-selective labeling.  SYTOX
Green is useful for 488nm laser line, and TO-PRO-3 will work with 647nm
laser line.  

For live cells, SYTO dyes will work, though their label pattern is a bit
variable depending on the dye and the cell type.  We at Molecular Probes
also just released DyeCycle Violet, a DNA label that excites with 405nm
diode as well as UV.  This will work for fixed cell labeling as well.

(-- I acknowledge corporate interests, etc. --)

Cheers,

Jason A. Kilgore
Molecular Probes / Invitrogen


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Zifu Wang
Sent: Tuesday, May 02, 2006 9:51 AM
To: [log in to unmask]
Subject: Re: DNA dye excited with visible laser

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Hello listers,

We have a Zeiss LSM 510 system without a UV laser or two-photon laser.
I
would like to learn your suggestions on which DNA dye excited by visible
laser (Argon or HeNe) is the best substitute of DAPI.

Thanks.

-------------------------
Zifu Wang, Ph.D.
Optical Biology Core Facility
Developmental Biology Center
2114 McGaugh Hall
Univerity of California
Irvine, CA 92697-2275
Tel:  (949) 824-3856
FAx:(949) 824-3571