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Just anecdotally, we've found that Atto425 works very well with the 405nm on our OMX, outperforming Pacific Blue, and Alexa 405 when conjugated to phalloidin.

Best,

Eric

On 28 Jun 2012, at 17:34, Kurt Thorn wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I haven't used any of these myself, but I've compiled a list of commercially 405nm excitable dyes for our users here:
> http://nic.ucsf.edu/dokuwiki/doku.php?id=dyes:405nm
>
> If you try any it would be great to get some feedback about how they perform.
>
> Best,
> Kurt
>
> On 6/28/2012 3:00 AM, Graham Wright wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Dear all,
>>
>> I'm looking for recommendations for a blue dye (ex. 405nm) to be used on a
>> secondary antibody. Unfortunately, the other wavelengths are already taken,
>> which would normally be my suggestion.
>>
>> I've had a look through the archives of this list and there is confirmation
>> that they are not ideal and tend to bleach easily, but are there any
>> updates on this since 2008/2010?
>>
>> Any comments and recommendations will be appreciated about the options:
>> Alexa 405, Pacific Blue, Cascade Blue...
>>
>> Much obliged,
>> Graham
>>
>


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