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Hi Andrew,



the camera-space numerical aperture is pretty low, about 0.06 (assuming 
field number of 22 mm and tube lens focal length 180 mm). It's even less for
smaller detectors... I don't think the low power lens could cause some 
aberrations, the microscope objective itself is the critical part.




For the same reason a flat piece of glass would just offset the image; the 
aberrations (not a pure astigmatism, in general) become important at much 
higher NA's.




You can try tilting a low power spherical singlet (plano-convex). It works 
well with diverging laser beam (NA 0.01), but I did not try some raytracing 
to see the exact behaviour in infinity space or in the converging beam just 
in front of the camera :-).




Of course translating a cylindrical lens closer to the camera reduces the 
astigmatism. It depends, what's easier for you to do mechanically - tilt a 
lens or shift it along it's axis...




Zdenek







---------- Původní zpráva ----------
Od: Andrew York <[log in to unmask]>
Komu: [log in to unmask]
Datum: 23. 4. 2014 23:20:49
Předmět: Re: cylindrical lenses with 10m focal length (1in diameter)

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This is off-topic, but what other methods have people used to introduce
controlled astigmatism? I've used a moderate focal-length cylindrical lens
directly in front of the camera for 3D localization, but our field of view
wasn't very big, and I never carefully checked that the astigmatism was
uniform across a large FOV.

Would a flat, tilted piece of glass in a non-infinity space be a better
way to achieve the same goal? If it works, you could tune the magnitude of
astigmatism by adjusting the tilt angle, and I'm guessing the astigmatism
would be more uniform across the field. Is this a bad idea, an old idea, or
something worth trying?


On Wed, Apr 23, 2014 at 1:38 PM, Dan Focht <[log in to unmask]> wrote:

> *****
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>
> Zulin
>
> What shape are you able to acquire the 1m Cyl lens.
> Tell me more about what is available and I will tell you what we can do
> with it.
>
> Dan
>
>
>
>
>
> On Apr 22, 2014, at 11:40 PM, Zulin Yu wrote:
>
> *****
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>
> Dear All,
> We are planning to add cylindrical lenses with 10m focal length to our
> microscopy to do 3D STORM. Unfortunately, I am having problem to find any
> round cylindrical lens (1 inch) with such a long FL. As far as I know, CVI
> is the
> only company making 10 m cylindrical lens, but they are not willing/able
> to cut
> their lenses to round shape (1 inch). It will be really helpful if anyone
> could tell me
> which company can make these lenses. Thanks a lot, Zulin
>
>
> Dan Focht
> Bioptechs, Inc.
> 3560 Beck Rd.
> Butler, PA 16002
> www.bioptechs.com
> P: (724)282-7145
> F: (724)282-0745
> [log in to unmask]
>"