***** To join or leave the confocal microscopy listserv or to change your email address, go to: https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1 Post images on http://www.imgur.com and include the link in your posting. ***** Fluorescent magic markers are great for turning chrome resolution targets into fluorescence targets. Mike On Mon, Jun 19, 2023 at 9:40 AM Khoroshyy Petro <[log in to unmask]> wrote: > > ***** > To join or leave the confocal microscopy listserv or to change your email address, go to: > https://urldefense.com/v3/__https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1__;!!CGUSO5OYRnA7CQ!Zgv1xms8a04_hrRN4GwYzfoVm2uAaH9B0rm3b3dRGSD2On63t0s19pmMyu5BykuJ1CCi88xABulGbOAsR7SSZ_DR$ > Post images on https://urldefense.com/v3/__http://www.imgur.com__;!!CGUSO5OYRnA7CQ!Zgv1xms8a04_hrRN4GwYzfoVm2uAaH9B0rm3b3dRGSD2On63t0s19pmMyu5BykuJ1CCi88xABulGbOAsR4pE7rpf$ and include the link in your posting. > ***** > > Hi Marie. > Thanks for answering, I have these, what I need is a bit different. > I have a cover slip with a scale made by evaporated metal. What I want is > to mount it with fluorescent mounting media and have in addition to > reflected and transmitted image, a fluorescent image as well. > Best. > Petro. > > > On Mon, 19 Jun 2023, 14:38 Held, Marie [mheldb], <[log in to unmask]> > wrote: > > > ***** > > To join or leave the confocal microscopy listserv or to change your email > > address, go to: > > https://urldefense.com/v3/__https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1__;!!CGUSO5OYRnA7CQ!Zgv1xms8a04_hrRN4GwYzfoVm2uAaH9B0rm3b3dRGSD2On63t0s19pmMyu5BykuJ1CCi88xABulGbOAsR7SSZ_DR$ > > Post images on https://urldefense.com/v3/__http://www.imgur.com__;!!CGUSO5OYRnA7CQ!Zgv1xms8a04_hrRN4GwYzfoVm2uAaH9B0rm3b3dRGSD2On63t0s19pmMyu5BykuJ1CCi88xABulGbOAsR4pE7rpf$ and include the link in your posting. > > ***** > > > > Hi Petro, > > > > Would these do the job? > > https://urldefense.com/v3/__https://www.chroma.com/products/accessories/92001-autofluorescent-plastic-slides__;!!CGUSO5OYRnA7CQ!Zgv1xms8a04_hrRN4GwYzfoVm2uAaH9B0rm3b3dRGSD2On63t0s19pmMyu5BykuJ1CCi88xABulGbOAsR2m6NwTL$ > > No association with the company but we use those when we need to image a > > homogeneous sample. 😊 > > > > Annual leave coming up: 24/07 – 09/08 > > Best wishes, > > > > Marie > > > > Thank you for also reading this bit: > > Acknowledgement in publications and presentations of CCI performed work is > > vital to secure support and funding necessary to maintain this valuable > > research resource. For publications that include work performed in the > > facility, please use the acknowledgement statement "We acknowledge the > > Liverpool Centre for Cell Imaging (CCI) for provision of imaging equipment > > and technical assistance." and include required grant numbers as listed > > here https://urldefense.com/v3/__https://cci.liv.ac.uk/facility.html__;!!CGUSO5OYRnA7CQ!Zgv1xms8a04_hrRN4GwYzfoVm2uAaH9B0rm3b3dRGSD2On63t0s19pmMyu5BykuJ1CCi88xABulGbOAsRwUMJuq8$ . Please also consider staff for > > a named acknowledgement mention or even co-authorship if they played a key > > role in the study. > > > > -----Original Message----- > > From: Confocal Microscopy List <[log in to unmask]> On > > Behalf Of Khoroshyy Petro > > Sent: 19 June 2023 10:46 > > To: [log in to unmask] > > Subject: Dye mixture to prepare fluorescent slide > > > > ***** > > To join or leave the confocal microscopy listserv or to change your email > > address, go to: > > https://urldefense.com/v3/__https://lists.umn.edu/cgi-bin/wa?SUBED1=confocalmicroscopy&A=1__;!!CGUSO5OYRnA7CQ!Zgv1xms8a04_hrRN4GwYzfoVm2uAaH9B0rm3b3dRGSD2On63t0s19pmMyu5BykuJ1CCi88xABulGbOAsR7SSZ_DR$ > > Post images on https://urldefense.com/v3/__http://www.imgur.com__;!!CGUSO5OYRnA7CQ!Zgv1xms8a04_hrRN4GwYzfoVm2uAaH9B0rm3b3dRGSD2On63t0s19pmMyu5BykuJ1CCi88xABulGbOAsR4pE7rpf$ and include the link in your posting. > > ***** > > > > Hi. > > Has anyone tried to mix dyes with mounting media to prepare homogenous > > fluorescent slide? Any recommendations on which days to try? I would start > > with rhodamine and fluoresceine or some ATTO dyes. > > Any recommendations for a dye in blue region. > > Best. > > Petro. > >
