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Hello group,

What would be the best test to check laser alignment? Beads? How do
manufactures perform alignment procedures?  We have two fibers one for
405 and the other for 488 , 543, 633 lines and I feel that 405 laser
is not aligned correctly. Our service rep would simply move the
collimator for the 405 to the point where everything looked ok but
that was rather subjective. The worst thing is that now we have new
settings for the collimator but people reuse settings from old images
and revert back to wrong collimator position?  Any good way to solve
this problem?

Thank you,

Daniel F

On 7/14/07, Femto @Sciner: Del Mar Photonics <[log in to unmask]> wrote:
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> >Why is it so popular?
>
> It's possible to use multiple lasers without alignment problems, right?
>
> Best regards,
> Sergey Egorov
> Del Mar Photonics, Inc.
> 4119 Twilight Ridge
> San Diego, CA 92130
> tel: (858) 876-3133
> fax: (858) 630-2376
> [log in to unmask]
> http://www.dmphotonics.com/
>
> ----- Original Message -----
> From: Steve Baer
> To: [log in to unmask]
> Sent: Friday, July 13, 2007 9:56 PM
> Subject: Multiple fiber laser inputs – some questions
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Hi everyone,
>
> As best as I can tell, no manufacturer sold a confocal/multiphoton
> microscope with more than a single fiber input before about 1996. Then
> Leica, Zeiss and Olympus began almost simultaneously to offer this feature.
> Who first offered this feature commercially and when? Why is it so popular?
>
>
> Thanks.
>
>
> Steve


-- 
Daniel Fimiarz
CCNY Laser Scanning Microscopy Laboratory, J525
(212) 650 8586
www.ccnyconfocal.org