CONFOCALMICROSCOPY Archives

October 2001

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Subject:
ECFP & EYFP
From:
Tom Gore <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 10 Oct 2001 09:37:38 -0700
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Has anyone had success in discriminating between ECFP (excitation at 433,
emission at 475) and EYFP (excitation at 513, emission at 527) using a
Zeiss LSM 410?

If so what filter combinations seemed to work best for confocal imaging?
_____________________________________
Tom Gore, Advanced Imaging Laboratory
Biology Department, University of Victoria
Box 3020, Station CSC,
Victoria, BC, V8W 3N5 Canada
voice (250) 721-7134   fax (250) 721-7120
web:  http://web.uvic.ca/ail/

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