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Hi Juan Luis

Have you tried DRAQ5 from Biostatus, UK. http://www.biostatus.com/product/draq5/spectral_profile/

You will need a red laser (633,638,647), emission in the 675nm for best results. If you don't have a red laser, the 568 line from ArKr will work. Draq5 works very well on Leishmania.

Hope that's help

Marcel

Marcel Desrosiers
Coodonnateur / Manager

Laboratoire commun de Cytométrie et d'Imagerie Numérique /
Cytometry and Digital Imaging Core Laboratory
INRS-Institut Armand Frappier
Institut National de la Recherche Scientifique
Université du Québec

Adresse / address : 531 Blvd Des Prairies
                               Laval, Québec
                               Canada, H7V 1B7
Courriel / E-mail : marcel.desrosiers @ iaf.inrs.ca
Tél :  (450) 687-5010 poste/ext. 4314
Fax : (450) 686-5501





-----Message d'origine-----
De : Confocal Microscopy List [mailto:[log in to unmask]] De la part de Juan Luis Ribas
Envoyé : 22 novembre 2006 03:15
À : [log in to unmask]
Objet : Staining DNA in Cy5 range

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Hello everybody, I'm interested in staining nuclei of trypanosomas with 
some substance similar to DAPI but that works in the range of Cy5. This 
is because my confocal doesn't have diode 405 or UV laser. If someone 
could help me I would be very greatfull.
Best regards.

Juan Luis
--------------------------------------------------------------------------
Dr. Juan Luis Ribas Salgueiro
Servicio de Microscopía
Centro de Investigación, Tecnología e Innovación.
Universidad de Sevilla.
Av. Reina Mercedes s/n 41012 Sevilla
Spain