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Hello,

We use blue ink (permanent marker) on a coverslip with a small drop of water on the slide. You can focus de ink and bleach it.

Regards,
Katherina



El 12/01/14, Anton Kamnev  <[log in to unmask]> escribió:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
> 
> Dear all,
> 
> In our LM core we face a problem of frequent calibration of our "FRAP"
> devices. Although the calibration itself is quite fast, many users just
> don't have good samples which could be used for this purpose.
> 
> Therefore, I was wondering if there are any commercial products for
> calibration of bleaching devices? What I'm looking for is a slide with a
> thin uniform layer of a dye which could be bleached. The dye should be
> immobilized so a bleaching pattern would be observable for some period of
> time after the bleaching.
> 
> I would really appreciate if you could share some experience about samples
> you use for calibration of bleaching patterns.
> 
> Although I'm primarily interested in commercial solutions, I would very much
> appreciate some protocols for home-made calibration slides as well.
> 
> Thanks in advance!
> Anton.
> 
> ---------------------------
> Anton Kamnev, PhD
> Imaging Manager
> Mechanochemical Cell Biology Building
> Division of Biomedical Cell Biology
> Warwick Medical School
> The University of Warwick
> Coventry, CV4 7AL UK
> 
> tel: +44 (0) 24-7615-1934
> cell: +44 (0) 782-408-6941
> email: [log in to unmask]
> 
> Divisional Secretary: Karl Waldron
> Tel: +44(0) 24-7615-1154
> [log in to unmask]
-- 
---------------------------------------------------------------
Katherina García García
Técnico Especialista en Microscopía y Citometría
Tlf. 954977432
[log in to unmask]
Centro Andaluz de Biología del Desarrollo
Consejo Superior de Investigaciones Científicas
Universidad Pablo Olavide
Ctra. Utrera km1
41013 Sevilla
España