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I wondered if anyone out there has had any experience with Tsien's  
(now INvitrogen) Flash tag system.  We made our first construct  
recently containing the tetracysteine motif, but got no fluorescence  
at all with the biarsenical compound.  We are checking expression  
now. The absence of much literature of this outside of the Tsien lab  
has me concerned that there are issues with making it work.
Dr. David Knecht
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)