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dear all,

i am currently doing antibody stainings on paraffin sections.
unfortunately, i am always getting very bright dots when i look at
the staining under the confocal.when i do the control without first
antibody, i also see these dots. therefore, they should clearly come
from the 2nd antibody. it does not matter whether i use an alexa 488
or alexa 546 coupled antibody, they both produce these effects. maybe
the antibodies are bad, but they are from a commercial source and
work fine in many applications. so, is this a probelm related to
paraffin sectioning?

i would appreciate any ideas or experience how to get around that
problem and be forever grateful!


best wishes,

Florian
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Florian Ulrich
Heisenberg Lab
Max-Planck-Institute for Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 Dresden
Germany
phone: (+49) 351 210 2689
fax:    (+49) 351 210 1489
email: [log in to unmask]