Hello, all,
I'm trying to quantitatively image Ca2+ on pancreatic beta cells with
INDO-1
using a NIKON RCM8000 confocal microscope. The first problem I met is the
shading correction. For a homogeneous sample, one side of the imaging
field always gives higher intensities than the other side on my setup. I'm
wonderring if other microscope will also have similar problems.

I tried to do the shading correction, however I still cann't get a
homegeous image after the shading correction. I will be appreciated if
anybody on the list could give me some suggestions. Thanks in advance.

Best wishes!

Weijun

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Weijun Qian
Department of Chemistry, P.O. Box 117200
Gainesville, 32611-7200
Phone: 352-846-5462(H); 352-846-0838(O)