>In my experience, the best fixative for fluorescence immunocytochemistry is
>paraformaldehyde. We use it at 4%, freshly prepared from powder (Merck
>4005-1000), in Na phosphate buffer. You should be careful about final pH
>(7.2-7.4) and adapt the molarity of the buffer to your biological material
>(usually between 0.15 and 0.2 M).

This depends on the application, of course. Other fixation methods to try
are
glutaraldehyde
glutaraldehyde/paraformaldehyde
methanol at -70degC
EGS

Always compare specimens fixed with at least 2 methods, otherwise you
might be up to a bad surprise later.

just my $0.02

blu



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