Hello whomever you are,

Cell Tak is essentially a glue.  If you don't block the exposed material, most anything will stick, including dyes.  Do a blocking incubation like you would for immunostaining or Western blot.  Also, it pays to remember that this material, much like other adhesive coatings, may alter the natural morphology and physiology of cells because 1) they are cemented to the surface and cannot move as freely as in vivo and 2) surface receptors are not interacting with  natural substrates.  
C


Carl A. Boswell, Ph.D.
Molecular and Cellular Biology
Univ. of Arizona
520-954-7053
FAX 520-621-3709


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of SUBSCRIBE CONFOCALMICROSCOPY Estelle Chiari
Sent: Wednesday, February 16, 2011 7:06 AM
To: [log in to unmask]
Subject: Re: attachment of primary T cells

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We tried the BD cell tak product, cells are very well attached but we  have trouble to figure out if the picture fussiness (DAPI is horrendous) are due do the mounting media reacting with the cell tak or if cell tak isr eally not good for confocal...