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>
>This is why it is good to sample a little higher than the Nyqyist
>rate.  Airy discs can be separated from Poisson noise by deconvolution
>or by other noise reduction algorithms.
>
>Brian

Hi Brian,

I agree that sampling a bit higher than Nyquist never hurts, 
especially if you deconvolve (as you always should), but I think that 
it is a mistake to think that one can "separate" out the noise by 
decon. I think that noise is pretty fundamental.

Would you not agree that the best you can do is effectively average 
the statistical noise over all the measurements needed to define a 
PSF at the sampling you are using? If we assume that from 64 to 125 
pixels are needed to define a PSF, this produces a significant noise 
reduction. But not an infinite one.

Best

Jim Pawley
-- 
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Prof. James B. Pawley,               		            Ph. 
608-238-3953              	           
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3D Microscopy of Living Cells Course, June 10-22, 2012, UBC, Vancouver Canada
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	       "If it ain't diffraction, it must be statistics." Anon.