"John N. Wright" <[log in to unmask]> writes
>Our cardiomyocyte cell cultures, grown on gelatin coated coverslips
>and fixed in 3% paraformaldehyde are lifting off the coverslips after a
>30 min./4=B0C treatment with Na Borohydride.  Can anyone suggest a more
>gentle quenching protocol to solve this problem without significantly
>reducing antigenicity.
 
according to the local expert
*the short answer is that the quenching referred to is only needed if
*using glutaraldehyde, not fpormaldehyde, so they dont need to do it in
*the first place...it will have no effect as it serves only to reduce
*double bonds of the schiff base, and wont influence the methylene bridges
*that formaldehyde generates.
 
 
cheers,
Danny Thomas  <[log in to unmask]>