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Can I please make it clear that neither I, nor Nuno, nor other contributors who have mentioned other people, grudge these awards in the slightest. The three winners (and I have known Stefan Hell for 30 years) all totally deserve their awards. What's more, it is a boost for all of us that super-resolution microscopy has made the Nobel. But as with many other cases in the past, the strait-jacket of only 3 winners (max) means that other deserving people miss out. This is particularly the case where two topics share the same prize. (cf EM and STM some years ago). So, while congratulating the three winners, it is good that we recall other contributors, so that they will know that even if they didn't get the prize, they are still remembered by the community.
Guy
Guy Cox, Honorary Associate Professor
School of Medical Sciences
Australian Centre for Microscopy and Microanalysis,
Madsen, F09, University of Sydney, NSW 2006
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Andreas Schönle
Sent: Friday, 10 October 2014 7:25 PM
To: [log in to unmask]
Subject: Re: congratulations to Eric, Stefan and W.E. for Nobel Prize in Chemistry, for the development of super-resolved fluorescence microscopy
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Dear Guy, Nuno, dear list members!
Surely this discussion will now go on for a while but I would like to set a few facts straight, so it can be put on solid ground.
1)
Stefan Hell was never a student of Chirstoph Cremer. His PhD advisor was Prof. S. Hunklinger and both 4pi and STED microscopy were conceived by him independently. The principle of STED is completely unrelated to 4pi and 4pi has nothing to do with what the Nobel prize was awarded for (see below).
2)
4pi microscopy is not a super-resolution method (it is diffraction limited).
Christoph Cremer's original proposal to use light coming from all directions was aimed at increasing lateral resolution and was based on the wrong assumption that inverting the light-field of a point source <<lambda by means of a hologram could beat the diffraction limit.
(this neglects the fact that the near field decays exponentially and that this cannot be inverted in conventional materials)
3)
Localization is not resolution. Impressive work was done localizing single or several light sources separable by spectral properties, stepwise bleaching etc. - usually with the goal of measuring distances. But dense images can (as of now) only be obtained by time-sequentially switching molecules on and off.
This was first realized for ensembles in STED and molecule-by-molecule in PALM/STORM/FPALM.
Again, this is not to diminish anybody's work in the field. Over the years I have read many articles describing important and inspiring work. And I wholeheartedly agree that the field would not be where it is without these contributions.
But when discussing who should be honored for finding the key to circumvent the diffraction limit in optical imaging, it is important to identify those people that had the right idea, recognized its importance and proved this by actually putting it to work.
Best regards,
Andreas
--
Andreas Schoenle, Dr.
Abberior Instruments GmbH, D-37077 Goettingen, Germany
phone: +49 (551) 30724170
fax: +49 (551) 30724171
http://www.abberior-instruments.com
mailto:[log in to unmask]
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Nuno Moreno
Sent: Freitag, 10. Oktober 2014 00:19
To: [log in to unmask]
Subject: Re: congratulations to Eric, Stefan and W . E. for Nob el P rize in Chemistry, ³ for t he development of super-reso lved fluores cenc e mi croscopy²
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Dear Guy
You're absolutely right. I actually sent an internal email (for the
institute) a few hours after the announcement with, among other stuff, this:
"As always there are many others that should be on the laureates list but one needed the final click. In my opinion Cremer from Heidelberg university should be one of them ...."
Nuno Moreno
On 09 Oct 2014, at 02:16, Guy Cox <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> The three names is always a problem - especially when the prize is
> awarded
essentially for two different techniques. Christoph Cremer (Stefan's
supervisor) could well also feel he'd been passed over.
>
> Guy
>
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