CONFOCALMICROSCOPY Archives

June 1998

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Subject:
From:
"Steve Gaffin, Ph.D." <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Mon, 22 Jun 1998 12:23:31 EST
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While not exactly what you wanted, I wanted to study cells at elevated
temperatures and wanted a cell with circulating water.  I went to a local high
school which teaches operation of lathes and milling machines.  I told them what
I wanted, gave them some plastic and they were delerious with joy to design and
machine for me the desired cell in 3 days, without charge, and using their best
3rd and 4th year students.  For them this was much better didactically than
machining some more candle sticks.
We routinely use the cells at both high (60oC) and low (0oC) temps.  Works
great!
Good luck.

Steve Gaffin
US Army Research Institute for Environmental Medicine
Natick MA 01702
Tel 508-233-4867
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Subject: Bioptechs objective heater/INSTEC warm stage
Author:  Confocal Microscopy List <[log in to unmask]> at
Internet-Mail
Date:    6/22/98 12:10 PM


Hi - I was wondering what people thought of the Bioptechs objective
heater. I would like to use it in combination with an INSTEC WS60
Microscope Warm Stage System to keep my cells at 37deg on an inverted
microscope. I view my cells by placing the glass coverslips into
reusuable metal chambers (about the size of a Petri dish) purchased from
Molecular Probes.
    If anyone has had experience with the Bioptechs objective heaters
and/or the INSTEC warm stage and wouldn't mind sharing it, I would really
appreciate it. Thanks in advance!

Mary Teruel
[log in to unmask]

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