***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** ** Commercial reply ** Hi, Joe, I'm wondering how the results were disappointing. I'd be happy to troubleshoot offline. But what I can add is that violet dyes in general tend to be dimmer and less photostable than many other wavelengths. In my testing, I have found the DyLight 405 is brighter and a bit more photostable than Alexa Fluor 405 when compared side-by-side, if that might help. Jason Jason A. Kilgore Technical Application Scientist Molecular Probes / EVOS Tech Support Life Sciences Solutions [log in to unmask] 541-335-0353 - Tech Support -----Original Message----- From: Confocal Microscopy List <[log in to unmask]> On Behalf Of Joe Lebowitz Sent: Thursday, May 2, 2019 1:17 PM To: [log in to unmask] Subject: Well-liked 405 secondary antibodies CAUTION: This email originated from outside of the organization. Do not click links or open attachments unless you recognize the sender and know the content is safe. ***** To join, leave or search the confocal microscopy listserv, go to: https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=DwICAg&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=ernFKocE5VPUm_dilkkhh5hlRR5IZ-nSMDwol0CSJrw&s=RrwAIVBWZw4Zco3-pik7MFS9Y0fFYIi_mHgr17YbGLw&e= Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=DwICAg&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=ernFKocE5VPUm_dilkkhh5hlRR5IZ-nSMDwol0CSJrw&s=8OzqrEqpgkSRL5eMkKqlJ79_bc_Y703CsW4FgnyQz9g&e= and include the link in your posting. ***** Hello all, We have had some reproducibly disappointing results with Alexa Fluor 405 tagged secondary antibodies. Does anyone have a go-to secondary in this color other than the AF? We are trying to multiplex as many channels as possible and this is the only one we struggle to get good brightness/SNR with. Thanks! joe -------------------------------- Joe Lebowitz Ph.D. Candidate NINDS Pre-doctoral Fellow Khoshbouei Lab UF Department of Neuroscience (561) 504-3810
