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I am interested in the 405 laser since I already have the UV optics and our
UV argon laser is coming to the end of its lifetime.  Does anybody know how
much the 405 laser costs?

Thanks,

Zifu


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Tony Collins
Sent: Wednesday, November 02, 2005 7:01 AM
To: [log in to unmask]
Subject: Re: Réf. : Adding a 405 laser t o LSM 510

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Just to expand the topic, has anybody swapped their (expensive, hot) UV 
Argon laser for a (much cheaper, and cooler) 405 nm laser? The UV optics 
are already there so is it just a case of swapping filter sets and 
aligning? Would the AOTF need changing/recalibrating?

Aside from running costs, would spherical and chromatic abberrations be 
reduced significantly at 405nm? We have a Zeiss 63x 1.4 NA plan-apo 
objective with a shocking UV hotspot that Zeiss can't seem to "tweak" 
away except by opening the pinhole wide.

Tony

Alison North wrote:

> Search the CONFOCAL archive at 
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Hi John,
>
> Louis is absolutely right - I had the 405 laser retrofitted to my 
> confocal about 18 months ago and we did have to send the whole system 
> back to Germany to add in the UV optics.  And be warned - they said it 
> would take 2 months but it was actually gone for 4 months.
> Having said that, everybody absolutely loves it now and we've had no 
> problems at all with the laser.
>
> Best wishes,
> Alison
>
> Louis Villeneuve wrote:
>
>> Hi John,
>>
>> I'm using a LSM 510 confocal bought  in 2000.  I have an Argon 
>> (458-488-514nm), HENE1(543nm) and HeNe2(633nm).  In order to add a UV 
>> excitation line, I have been told that the microscope have to be send 
>> back in Germany for realignment and ajustment. The laser won't be a 
>> problem but time without using the scope could be one!  You can 
>> consider also buying new filters in order to accomodate to your new 
>> wavelenght...
>>
>> Have a nice day!
>>
>> Louis
>>
>>

-- 
Tony Collins, Ph.D.
Facility Manager
Wright Cell Imaging Facility
Toronto Western Research Institute
13-407 McLaughlin Pavilion
399 Bathurst Street
Toronto, ON. M5T 2S8
tel. (416) 603 5367 fax: (416) 603 5745
http://www.uhnresearch.ca/wcif