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Hi

I use DABO in a formulation of 1% DABCO in 85 - 90 %  glycerol, with 10
- 15 % PBS  if I use FITC I adjust the pH to 8.0 - 8.5 using indicator
strips, as FITC is noticably brighter at a higher pH.

Russ



Russell N. Spear
Sr. Research Specialist
Dept. of Plant Pathology
Univ. of Wisconsin
1630 Linden Dr.
Madison WI 53706

voice 608.263.2093
fax     608.263.2626

>>> Louis Villeneuve <[log in to unmask]> 03/11/08 8:14 AM
>>>
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Hi everyone,

I am looking for a recipe for mounting media with DABCO diluted in 
glycerol.  The one I have is DABCO, 0.4% (diluted in PBS) mix 1:4 in 
glycerol.  Also, where I could find a database for finding the
refractive 
index of several mouting medium/media?

Thanks

Louis Villeneuve

Louis Villeneuve
Research Associate - Confocal Microscopy
Heart Montreal Institute- Research Center
5000 East Belanger
Montreal (Qc), Canada
H1T 1C8

514-376-3330 ext 3511
514-376-1355 (Fax)

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