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November 2000

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Confocal Microscopy List <[log in to unmask]>
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Sun, 19 Nov 2000 07:17:00 -0800
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I went to the site. It sounds like a nice piece of software, but no price
information at all. What do you mean by expensive?

----- Original Message -----
From: Aryeh Weiss <[log in to unmask]>
Newsgroups: bit.listserv.confocal
To: <[log in to unmask]>
Sent: Sunday, November 19, 2000 4:46 AM
Subject: Re: Analyzing filopodia


> F Javier Diez-Guerra wrote:
> >
> > We are investigating the intracellular mechanisms underlying filopodia
> > induction and extension in cultured cells and would like to know if
there
> > is any software available that could help us with the analysis. At the
> > moment, we are counting manually the number and length of newly formed
> > filopodia, but this task is time-consuming and somehow biased by our
> > expectations.
> >
>
> Image Pro Plus (Media Cybernetics, www.mediacy.com) has a number of
> tools that can be applied to this problem.
> It is not free (let's rephrase that -- it is expensive), but very
> convenient and stable. There mailing list and support are excellent, so
> at least you get what you pay for.  There is a nice set of example
> macros, some of which might give you useful ideas. You should also check
> the various object measurements in the object counting tool, and
> consider using morphological operators to segment your filopodia (the
> skeleton operator might be useful)
>
> Image-J has also been suggested and it is a very nice package, but not
> as stable as IPP and not as simple. Depends if you have more time than
> money...
>
> Disclaimer: I don't get any kickbacks from Media Cybernetics, but I have
> a lot of experience with IPP so I tend to be biased.
>
> --aryeh
> --
> Aryeh Weiss                          | email: [log in to unmask]
> Department of Electronics            | URL:
> http://optics.jct.ac.il/~aryeh
> Jerusalem College of Technology      | phone: 972-2-6751146
> POB 16031                            | FAX:   972-2-6751275
> Jerusalem, Israel                    | ham radio: 4X1PB/KA1PB
>

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