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Here are two ways that we have looked at large methyl salicylate whole mounts using an inverted microscope.
1. Put in MatTek coverglass bottom dishes.
2. Bridge a microscope stage plate with a small hole with a large coverslip. Put a thin line of petroleum jelly or thick silicon equivalent around hole and press coverslip down to make a seal. Put tissue on coverslip immediately above hole.
In both cases, a slight weight may be put on the tissue to make it flatter without squishing too much.
Here's an example from circa 2000 using a BioRad Radiance 2000: http://www.flickr.com/photos/mcammer/3759826359/
Regards,
Michael
________________________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208 Cell: (914) 309-3270
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Yevgeniy Romin
Sent: Monday, January 14, 2013 3:04 PM
To: [log in to unmask]
Subject: BABB and Methyl Salicylate
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Dear Listers
Our lab is working on clearing thick sections for confocal imaging and we have had great success with BABB as well as Methyl Salicylate. We are currently looking for a way to seal a tissue submerged in these reagents underneath a coverslip to make it easier to image, since both of these reagents are very corrosive and could be harmful to the optics as well as the user. We haven't been able to successfully seal these slides. Has anybody here had any successful experience with sealing slides with these reagents? It would need to be something that can resist the corrosive nature of these reagents.
Thanks to all of you in advance,
Yevgeniy
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