It should be possible to do this just with the normal software, if you have the newest version.
in the bleach menu where you can define the region of interest it is possible to mark the option.
 
repeat bleach after # of scans
 
Then it should be easy.
 
Joost W

	-----Original Message----- 
	From: Confocal Microscopy List on behalf of Alison North 
	Sent: Wed 20-10-2004 18:29 
	To: [log in to unmask] 
	Cc: 
	Subject: Re: FLIP imaging
	
	

	Search the CONFOCAL archive at
	http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
	
	Hi Roger,
	You can certainly do FLIP using the Multitime macro of the Zeiss LSM
	software.  Do you have this?
	Hope so...
	Alison
	
	At 02:42 PM 10/19/2004 -0700, you wrote:
	>Search the CONFOCAL archive at
	>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
	>
	>I have a techniques question from a user.  We are using Zeiss LSM 510
	>(v. 3.x ? Software) Vis system.  He wants to do FLIP imaging, but has
	>not been able to repeatedly bleach and scan.  I have not done any FLIP
	>and am having trouble understanding exactly what he is trying to do.
	>Does this technique require use of a Macro on the Zeiss system?  Thanks
	>for any tips.
	>
	>Roger H. Adamson, PhD
	>Physiology & Membrane Biology
	>University of California
	>One Shields Avenue
	>Davis CA USA
	>95616-8644
	>
	>[log in to unmask]
	>530-752-2180 (phone)
	>530-752-5423 (fax)
	
	Dr. Alison J. North
	Rockefeller University,
	Bio-Imaging Resource Center, Box #209,
	1230 York Avenue,
	New York,
	NY 10021,
	USA
	
	Tel. (212) 327 7488
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