I've been working lately on confocal microscopy with very volatile and 
toxic organics.  The chambers described today are quite useful 
(Matek...) for cell culture and cell imaging; however, the solvents I've 
been working with lately readily attack plastic and rubber.  I would 
need a chamber made of glass or fused silica, with a thickness at the 
bottom comparable to a 0, 1, or 1.5 standard microscope coverslip for an 
inverted microscope, and Luer Locks or similar pipette access ports made 
of Teflon or with Teflon plugs.  I haven't found a supplier yet, if any 
vendor here or any of you know of a vendor that makes any type of 
chamber with glass only and with PTFE plugs that would be terrific.

Best,
Evangelos
Harvard CNS