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February 2012

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Subject:
Imaris and volume measurement
From:
Jean-Pierre CLAMME <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 16 Feb 2012 16:51:23 -0800
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Hi,

I was wondering if anyone knows what Imaris volume measurement statistic 
means in the case were the volume is not totally closed ?
Indeed I have cells stained with a membrane marker so I can create a 3D 
volume of the cell. However the bottom of the cell touching the glass has 
areas where there is no staining (or not strong enough). Therfore Imaris 
doesn't close the volume.

Thank you

JP 

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Jean-Pierre CLAMME, PhD
Chief Scientist
Nitto Denko Technical
501 Via Del Monte
Oceanside, CA 92058
E-mail: [log in to unmask]
Phone: +760.435.7065

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