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Guy,
	Thanks. I do not have access to either multiphoton or a uv 
laser. I assume that with DAPI one or the other of those is required 
for a signal (irrespective of cell health). Is that not so?

	Tobias

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>My good friend and colleage Jose Feijo did this with
>DAPI using two-photon excitation.  I did the 3D
>reconstruction and we published the results in:
>
>Jose A Feijo & Guy Cox, 2001.  Visualisation of meiotic events in living
>anthers by means of two-photon microscopy.  Micron 32, 679-684
>
>I do think that multiphoton is a must for this from the
>point of view of maintaining cell viability.
>
>                                            Guy
>
>
>>>   I would like to hear from anyone who has had success imaging
>>>  mitotic chromatin in living plant cells,
>
>--
>Associate Professor Guy Cox
>Electron Microscope Unit,
>University of Sydney,
>NSW 2006, Australia
>
>Phone:+61 2 9351 3176    Fax:+61 2 9351 7682
>http://www.guycox.net


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