At 12:56 11/10/98 +0000, you wrote:
>About a year ago Steve Potter made an excelent review of the multiphoton
>conference. I was wondering if any such event took place this year, and if
>there are significant advances one should be aware of for a possible future
>purchase. I've highlighted a few points that still float on my mind from
>Steve's comments:
>
>(...)
>>1024, and for that reason is more complicated than a pure
>>multiphoton system needs to be, but they believe people are
>>not ready to abandon their confocals yet.  Well I, for one,
>>am ready!
>
>This is a point I would really like to know more, namely:
>
>1. Are there any 2-photon "only" systems on the market? My impression is
>that only Bio-Rad and Leica sell them, but integrated in their base
>confocal layout.
>

I WAS TOLD THAT ZEISS IS READY TO SELL FSEC BASED MULTI-PHOTON SYSTEM. ONE
THEY ARE GOING TO DELIVER IN USA NEXT MONTH. BUT THEY SOLD NUMBER OF UNITS
IN EUROPE. CONTACT ZEISS REPRESENTATIVE AND THE ZEISS PEOPLE CLAIM THAT
THEY HAVE THE FIBER OPTICALLY COUPLED COHERENT TI:SAPPHIRE/VERDI LASER TO
THEIR CONFOCAL COMPARED TO BIORAD OPTICALLY COUPLED LASER TO THE CONFOCAL
SCAN HEAD.

>
2. Also regarding these two brands, does any one have information on the
>direct comparison between the Bio-rad fsec and the Leica psec excitation
>modes? Both vendors provide absolute pro's of their choice, but it's hard
>to see how things compare in pratice.
>>detection.  BioRad says their NDS detector is in the works.
>

I DO NOT KNOW IT'S POSSIBLE TO GET THAT KIND OF QUANTITATIVE COMPARISON
SIDE BY SIDE NOW, PROBABLY AFTER COUPLE OF YEARS.

>
>3. are there any progress on this? How critical is this change?

>>(...) Coherent's Verdi, a 5W green solid-
>>(...) Spectra Physics offers the Millenia (similar if
>>not identical specs to Coherent's Verdi) and their Tsunami
>>Ti:sapph.  They also recently announced a 10W Millenia,
>>although I don't know if that is shipping yet.
>
>Well they're both on the market now, and I was wondering if there's more to
>be know from:
>
>4. Direct comparison between Millenia/Tsunami vs. Mira/Verdi
>5. Direct comparison between 5 and 10W Millenia
>

I USE COHERENT 5W VERDI AND IT'S COMPACT, WORKS WITHOUT ANY PROBLEMS. WITH
10W VERDI YOU MAY GET BETTER TUNING SPECTRUM AND MORE POWER. I DO NOT KNOW
10W PUMPING REQUIRED TO USE THE MULTI-PHOTON SYSTEM FOR BIOLOGICAL
APPLICATIONS, PROBABLY IT MAY HELP TO GET DEEP INTO THE TISSUE. WITH 5W
PUMPING I WAS ABLE TO GET THREE-PHOTON IMAGES FROM LIVING CELLS.

I DO NOT HAVE ANY EXPERIENCE WITH SPECTRA-PHYSICS LASERS AND I HOPE SOMEONE
WILL RESPOND TO YOUR REQUEST.

>
>I'm sure there's is more, but I'll wait for more input from others.
>Many thanks for any input
>Jose
>
>
>ooooooooooooooooooooooooooooooooooooooooooooooooooooooo
>o                                                     o
>o Jose' A. Feijo', Prof.                                      o
>o                                                     o
>o Dep. Biologia Vegetal, Fac.Ciencias Univ. Lisboa    o
>o Campo Grande, Ed.C2, P-1700 LISBOA, PORTUGAL        o
>o                                                     o
>o tel. +351.1.7500069, fax +351.1.7500048             o
>o e.mail: [log in to unmask]                         o
>o URL: www.fc.ul.pt/departs/biologia_vegetal/ejf.html o
>o                                                     o
>ooooooooooooooooooooooooooooooooooooooooooooooooooooooo
>
>

Ammasi Periasamy, Ph.D.
Director, Center for Cellular Imaging
University of Virginia
Department of Biology, Gilmer Hall (039)
Charlottesville, VA 22903
E-mail: [log in to unmask]
http://shiva.bio.virginia.edu/~cci
Tel: (804) 243-7602; Lab.: (804) 982-4869; Fax: (804) 982-5210