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Hi David,
Thanks! I checked out Viasho which led me to two USA distributors.
Here's two lasers and price that caught my eye (and hopefully will never
be pointed at mine or anyone else's):
http://www.lasernrg.com/diode-and-dpss-laser-modules.html
*20W/445nm laser module
<http://www.synchrovision.co.uk/product/179-20w-445nm-laser-module-kvant>*
EUR 6,356
*6.8W/637nm laser module
<http://www.synchrovision.co.uk/product/175-6-8w-637nm-laser-module-kvant>
*?EUR 10,625
(a bit weird that a USA distibutor web site would post prices in Euro's
... but the links are to a UK company).
and, can apparently buy online:
http://www.synchrovision.co.uk/product/175-6-8w-637nm-laser-module-kvant
6.8 Watts, even if 90% power loss getting to each objective lens, would
imply 680 mW for one microscope (CW STED depletion and/or CW 2-photon
excitation of DAPI), or 68 mW for 10 scopes (and $1065 per laser,
ignoring splitters, intensity control - and safety goggles).
I am interested in setting up an instrument to ablate single cells.
20W/445nm should do it, though I might be concerned with also ablating
the optics and ceiling (latter assuming an inverted stand).
Another reason I am thinking about relatively low cost lasers is the
potential to provide light to new nanoscope(s), such as ...
York ... Shroff 2012 "MSIM" paper in Nature Methods:
For exciting fluorescence, two lasers were used: a 150-mW, 561-nm laser
(561, Coherent, Sapphire 561-150 CW CDRH) and a 200-mW, 488-nm laser
(488, Coherent, Sapphire 488-200 CDRH). Mechanical shutters (Thorlabs,
SH05 and SC10) placed after each laser were used to control
illumination. Beams were combined with a dichroic mirror (DC, Chroma,
525dcxru) and expanded 6.7× with a beam expander constructed from two
achromatic lenses (Edmund, f = 30 mm, NT49-352-INK and Thorlabs, f = 200
mm, AC254-200-A-ML). Expanded beams were directed onto a DMD (Digital
Light Innovations, D4100 DLP 0.55" XGA) 24 degrees off normal, so that
in the 'on' position the micromirrors tilted the output beam normal to
the DMD face. The central order of the resulting pattern was demagnified
1.5× with a beam de-expander (Thorlabs, f = 75 mm, AC254-075-A-ML and f
= 50 mm, AC254-050-A-ML), aligned in a 4f configuration such that the
DMD face was reimaged at the back focal plane of a 180 mm tube lens
internal to the microscope (Olympus, IX81).
Probably would not need multi-Watts, so something like:
*200mW/405nm laser module
<http://www.synchrovision.co.uk/product/87-200mw-405nm-laser-module-kvant>*EUR
770 (100 mW is EUR462, but why bother)
*200mW/445nm laser module
<http://www.synchrovision.co.uk/product/78-200mw-445nm-laser-module-kvant>*EUR
540
*300mW/532nm laser module
<http://www.synchrovision.co.uk/product/76-300mw-532nm-laser-module-kvant>*EUR
739
*170mW/637nm laser module
<http://www.synchrovision.co.uk/product/71-170mw-637nm-laser-module-kvant>*EUR
406 or *150mW/642 (640) nm laser module
<http://www.synchrovision.co.uk/product/61-150mw-642-640-nm-laser-module-kvant>*EUR
394
Or, Xiaowei Zhuang's latest - eight standard organelle dyes are
STORM-able,
http://www.pnas.org/content/early/2012/08/06/1201882109.abstract
(open access article)
Sincerely,
George
-------- Original Message --------
Subject: Re: has anyone put these wickedlasers (or equivalent
price/power) on confocal, MSIM, single molecule localization etc scope?
Date: Sun, 19 Aug 2012 16:52:27 -0700 (PDT)
From: David Baddeley <[log in to unmask]>
Reply-To: David Baddeley <[log in to unmask]>
To: [log in to unmask] <[log in to unmask]>
I can't find any details about the actual design of the laser modules
the website, but I looked into getting a very similar 1W blue laser
pointer that was on offer at deal-extreme. It turned out that the module
was originally designed for laser projection/lighting applications and
was a number of separate lower wattage modules fabricated side by side
on the same die (like some high power LEDs). This type of laser is
unsuitable/less suitable for microscopy applications due to the extended
source area and corresponding inability to be focussed down to a small
spot.
To switch / control intensity your best bet would probably be an
external shutter and/or filter wheel. We use viasho lasers which are
positioned, both in price/W and capabilities/quality, somewhere between
these and classical 'scientific' grade lasers. The viasho modules have
either TTL or analog control via bnc, but switching the lasers using
these inputs results in instability and mode hopping as they warm up and
cool down (I suspect the inputs are designed for more rapid modulation
on timescales less than the thermal relaxation of the laser die), making
shutters and a filter wheel the most practical method of control.
As to the glasses, they sell them separately as OD2 glasses without
specifying which of their lasers they should be used with, so I suspect
they've just specced them to be OD2 or better at all wavelengths of
laser they sell. This actually seems like a fairly responsible/
pragmatic approach for people selling a product like this.
cheers,
David
------------------------------------------------------------------------
*From:* George McNamara <[log in to unmask]>
*To:* [log in to unmask]
*Sent:* Sunday, 19 August 2012 1:23 AM
*Subject:* has anyone put these wickedlasers (or equivalent price/power)
on confocal, MSIM, single molecule localization etc scope?
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Dear listserv,
has anyone put these wickedlasers (or equivalent price/power) on
confocal, MSIM, single molecule localization etc scope (and figured out
how to control on/off, intensity, from USB or other port)?
http://www.wickedlasers.com/krypton?utm_source=Wicked+Lasers+New&utm_campaign=bd89072d39-S3_August_20128_17_2012&utm_medium=email
<http://www.wickedlasers.com/krypton?utm_source=Wicked+Lasers+New&utm_campaign=bd89072d39-S3_August_20128_17_2012&utm_medium=email>
532 nm, 750 mW, $999.95, Spyder Krypton
445 nm, 1250 mW, $399.95, Spyder Arctic
If you have, web page or other instructions, on how would be great.
George
p.s. if someone can explain to me (without cheating by asking the
company) the choice of glasses in the home page video,
http://www.wickedlasers.com/index.php , that would be great. If you make
it through the first video, click on the Arctic Popcorn video link. And
of course, "do not look at laser with remaining eye".
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