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July 2014

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Subject:
Re: recommended distortion target for multiphoton?
From:
Christophe Leterrier <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 22 Jul 2014 23:06:59 +0200
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*****
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Hi Michael,

If you have a good motorized stage, maybe you can scan one or a few beads
over the whole field of view and generate a "virtual" grid?

Christophe


2014-07-22 19:03 GMT+02:00 Michael Giacomelli <[log in to unmask]>:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Mark,
>
> I'm assuming you mean beads deposited in a grid or something similar?
> Could you point me to where you found this?
>
> Thanks,
> Mike
>
> On Tue, Jul 22, 2014 at 3:58 AM, Mark Cannell
> <[log in to unmask]> wrote:
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi Michael
> >
> > I’ve always used beads to test the microscope. They are relatively cheap
> and if you blow one up it don’t matter. As you say, evaporated metal
> targets are too easily damaged (as Jim Pauley and I discovered when testing
> my microscope back in ’95!)
> >
> > HTH
> >
> > Mark
> >
> > On 22/07/2014, at 4:30 am, Michael Giacomelli <[log in to unmask]> wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> Post images on http://www.imgur.com and include the link in your
> posting.
> >> *****
> >>
> >> Could someone recommend a good multiphoton distortion target?
> >> Preferably one that is relatively robust against damage.
> >>
> >> Currently I am using a thorlabs wire grid target with fluorophore
> >> behind it.  The beam is attenuated by the metal, resulting in an image
> >> of the grid.  However, passing through the entire 1 mm slide greatly
> >> reduces my resolution, and unless I am extremely careful, the grid is
> >> ablated by the beam.
> >>
> >> Thanks,
> >> Michael
> >
> >
> > Mark  B. Cannell Ph.D. FRSNZ
> > Professor of Cardiac Cell Biology
> > School of Physiology &  Pharmacology
> > Medical Sciences Building
> > University of Bristol
> > Bristol
> > BS8 1TD UK
> >
> > [log in to unmask]
>

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