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PI has a tradition in eukaryotic biology as an indicator of cell 
death through membrane disruption.  I am interested in whether any 
list watchers can tell me of cases in which strong PI staining is not 
an indicator of cell death.  If many examples of PI staining of 
growing (or growth-capable) cells exist, then of what is PI staining 
indicative other than a (transient) membrane permeability difference 
compared to untreated controls?
FYI, my definition of cell death is the inability of cells to divide 
when provided with appropriate conditions.  I am less interested in 
other measurements of viability - "dividing or dead" is my motto...
Thanks for your opinions.....
Rob Palmer
-- 
Robert J. Palmer Jr., Ph.D.
Natl Inst Dental Craniofacial Res - Natl Insts Health
Oral Infection and Immunity Branch
Bldg 30, Room 310
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396