CONFOCALMICROSCOPY Archives

November 1996

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Subject:
Cardiac fiber nuclei
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Confocal Microscopy List <[log in to unmask]>
Date:
Wed, 27 Nov 1996 17:11:59 -0200
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Dear Confocalists,

We are trying to stain cardiac fiber nuclei with two fluorescent dye:
YOYO-1 and Acridine-Orange. We have tried in non- and paraffin-embedded
sections. The problem is we are not getting a high contrast level
between the nuclei and the backgroung (fiber).
Does anybody have a similar problem?
Does anybody know a good solution for it?

Thank you very much.


Luiz Claudio Parzianello, E.E.
______________________________________
Heart Institute of Sao Paulo - HCFMUSP
    Division of Informatics - R&D
Av.Dr. Eneas de Carvalho Aguiar, 44
05403.900 Sao Paulo - SP - Brazil
Voice:  +55-11-3068.5311
Fax:    +55-11- 282.2354
E-mail: [log in to unmask]

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