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Would using an England Finder work?

Dave

At 07:22 AM 3/1/2007, you wrote:
>Hello All,
>
>We're taking some multimodal images of skin (SHG, 2P (auto), 
>reflectance, and Raman), 20-60x.
>
>The SHG, 2P, and reflectance are on one scope and the Raman is on 
>another (pretty cool images in all modes).
>
>We've reached a quandary on how to register these images (even get 
>near similar fields of view, which are between 50-250 um).
>
>A pen mark would be way too big.  Too many hair follicles to narrow 
>down to.  Latex beads will probably float away (the skin needs to 
>stay hydrated).  A grid coverslip? Maybe a gene gun?
>
>The scopes are in different buildings, so stability of the "mark" IS an issue.
>
>Any and all input is appreciated, again.
>Best,
>
>Gary

Dave McDonald
Scientific Imaging Lab
Fred Hutchinson Cancer Research Center
1100 Fairview Avenue North
DE-512
Seattle, WA 98109
206-667-4205