Hi

I wonder if anyone can help me find a way to put a figure on the
extent of a network formation.  I'm not too bothered about getting
an absolute figure so long as I can compare a number of samples
and get relative figures of the extent to which a network is formed in
each case.  I can get reasonably stained relatively thick specimens
and can then produce an optical stack using a confocal
microscope.  This allows me to visualise a relatively coarse 3D
network and visually I can classify the samples.  However I need to
convince those with less of 'an eye of faith' than I have that these
differences can be expressed as numbers using a less subjective
assessment.  I've had a look at some stereology papers related to
using an optical disector in a series of sections and can see how
to get a measure of numerical density of the relevant phase and a
measure of the number of 'objects' constituting that phase.  I'm not
sure how to distinguish between a sample with large isolated
particles and one with extended areas of network.  Anyone able to
help?

Regards

David Lewis


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