Or dihydroethidium - Molecular Probes


     Anna Smallcombe PhD      Applications (Bio-Rad)


______________________________ Reply Separator _________________________________
Subject: Re: DNA dye for living cells
Author:  Confocal Microscopy List <[log in to unmask]>  at
Internet
Date:    04/12/97 10:49


Why not trying ethidium bromide? it works with plant cells, is excited
over a broad range of wavelenghts (near UV, blue, even green) and
fluoresces in  orange-red.
Jean-Pierre Verbelen




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  J-P. Verbelen                              E-mail: [log in to unmask]
  University of Antwerp (UIA)                          Tel:+32-3-820-22-77
  Department of Biology                                Fax:+32-3-820-22-71
  Universiteitsplein 1       B-2610 Wilrijk
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On Tue, 2 Dec 1997, Amie E. Franklin wrote:

> Hi,
>
> Members of my lab want to perform living microscopy of yeast (pombe) cells
> expressing GFP and BFP fusion proteins. We are interested in viewing the
> chromatin and would like a fluorescent dye in another wavelength.
>
>
>
> thanks all for your help
>
> Amie Franklin
> [log in to unmask]
>