CONFOCALMICROSCOPY Archives

May 2014

CONFOCALMICROSCOPY@LISTS.UMN.EDU
Options: Use Monospaced Font
Show Text Part by Default
Show All Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Subject:
458 for GFP-RFP?
From:
"MODEL, MICHAEL" <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 6 May 2014 17:05:55 +0000
Content-Type:
text/plain
Parts/Attachments:
text/plain (12 lines) 
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Colleagues,

We need to buy optics to do mGFP-turbo RFP FRET, and it seems that excitation at 458 might give much less direct excitation of RFP with only little sacrifice in GFP excitation. But there might be some other consideration of which I am unaware. Has anyone had any good/bad experience using 458 line for GFP? Thanks in advance!

Mike Model

ATOM RSS1 RSS2