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Rachaei-
I don't know where you are writing from, but you can do anything with 
Advanced Visualization Systems software.  The learning curve is 
pretty steep and they may not have it everywhere.


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>Hi! We are trying to run an experiment that requires image acquisition of a
>very large area (a slice of rat brain; we use mosaic for this purpose),
>along with optical sectioning.
>
>However, the software that we are currently using does not allow us to
>acquire both mosaic and optical sectioning simultaneously due to the
>extremely large file size produced (about 2GB), which cannot be processed by
>the software (software limitation of file size is 200MB).
>
>I am wondering if it is possible for us to acquire the mosaic and optical
>sectioning separately (i.e. mosaic for every plane of interest), export out
>and compile them with another software. Does any of you have any suggestion
>of possible software that has the capability of compiling individual planes
>into a stack?
>
>Please advise. Thank you!
>
>Cheers,
>Rachael Lim

-- 
Carol A. Heckman, Ph.D.
Director, Center for Microscopy & Microanalysis
and Professor of Biological Sciences
Bowling Green State University
Bowling Green, OH 43403
USA
website: http://www.bgsu.edu/departments/biology/facilities/MnM