Hi
I'm interested in trying to correct for bleedthrough by subtracting a
proportion of the green channel signal (based on bleedthrough measured
with only a green label present) from the red channel.

Does this _have_ to be done using the same combination of gain & black
level settings for both images, the image in which bleedthrough is
quantitated, & the image I want to correct?  Is it possible to make this
procedure more useful, so that after measuring bleedthrough at one (or a
few) gain setting(s)for the two channels, I can calculate the correction
required for arbitrary gain settings if e.g. I keep black level constant?  (Is
there a simple relationship between gain and signal?)  I'm using a BioRad
1024, if it matters.

Thanks!
Richard Thrift
DepoTech Corp.
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