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Date: | Wed, 28 Feb 1996 13:55:45 GMT |
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hi,
just something i noticed the other day ....
If you use immersion media on a slide then try to view the sample
using a xz scan i have noticed that the z step is not quite correct.
I believe that the immersion fluid's viscocity cause the slide to
move slightly. This is quite a problem when registering images or
trying to get unblurred images with an xz scan. I am currently adding
stage clamps to the confocal stage to reduce this problem, has
anybody else had such problems ?
To check that this was the cause i taped the slide down with strong
sellotape, low and behold very little blurring in the z direction.
I wonder how many new microscopes have good stage clamps ?
Hope this has helped,
__________________________________________________________________
Steve Bagley
The Confocal Microscope Facility
Cells, Immunology and Development Voice: 0161 275 6771
University of Manchester Fax: 0161 275 3915
http://gonzo.sci.man.ac.uk/~sbagley/
If you tied buttered toast to the back of a cat and
dropped it from a height, what would happen ?
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