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* LiBH4 (same handling precautions as NaBH4 ... that is, avoid blowing 
up the lab or yourself) Radtke ... Germain 2020 PNAS 
www.pnas.org/cgi/doi/10.1073/pnas.2018488117 (note that AF594 and JoJo-1 
are relatively resistant).
* Gerdes 2013 PNAS www.pnas.org/cgi/doi/10.1073/pnas.1300136110 (see 
also their patents).
Akoya (ex-PerkinElmer protocols online).
*  UV irradiation ... shorter (wavelengths) is more energetic.

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Too bright is a good problem to have. Shorter exposure time (camera and 
LED) to limit intensity to within the dynamic range of the detector(s).

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spectral (aka multichannel) unmixing ... the flow cytometrists are now 
at over 40plex.

On 8/2/2021 7:22 PM, Chen Chin-Yi wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hello all,
> We have done multiplex staining on same samples by repeating rounds of immunofluorescence staining. Recently, we use Tyramine to enhance the signal with low abundant antigen. However, we have difficulty to get forward on next round of staining due to the strong enhancement of signals. We are wondering whether anyone how to elute/strip off the Tyramine amplified signals?
> We have tried some harsh method, such as 20%SDS+2ME @50C for 1.5hrs, to remove the Tyramine amplified signal; but it still remained on the slices.
> I would be very appreciated if someone with experience is willing sharing it with us.
> Thank you so much in advance.
> Best,
> Chin-Yi
>